Flow Cytometry and Sorting Review

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(More customer reviews)As a student working in the area of applied optics as related to flow cytometry, I had many questions to ask about the principles of operation and aplication of flow cytometry.
This text provided a wealth of knowledge in the field, from historical accounts of flow, through general principles of operation and overviews of technoligical developments and commercial instruments, to the biological application of these instruments to clinical and research laboratories.
'Flow Cytometry & Sorting' is excellent both as an introductory text and reference guide for flow cytometer developers, users, and researchers.

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Revised and updated, the second edition of this text describes and evaluates - in greater detail than the first edition - the most recent practical applications of flow cytometry technique to basic cellular biological investigations and clinical research on human neoplasms. Ideal for the experienced researcher as well as the novice, this informative book offers state-of-the-art reviews of all aspects of flow cytometry including instrumentation, cell preparation and staining, applications in cell biology, and more. New articles highlight investigations of higher plants, the flow cytometry of microorganisms, and measurements of intracellular ionized calcium and membrane potential, illustrating the techniques of specimen preparation, measurement and analysis for each. New chapters examine applications of flow cytometry to medical genetics, genetic toxicology and ultrasensitive analysis of molecules in solution. The second edition goes beyond the traditional analysis of DNA histograms with BrdU incorporation and DNA denaturability to identify and analyze the cell cycle more precisely.New or rewritten chapters discuss the importance of cytometry for measurements of nucleic acids, chromatin and DNA, and cover the cytometry of sperm and the cytopathic effects of viruses.

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